What is imidazole?

1. What is imidazole used for?
2. Which drug contains imidazole?
3. What are imidazole derivatives?
4. Why is imidazole aromatic?
5. What is the example of Diazole?
6. Why do we use imidazole in protein purification?
7. Who discovered imidazole?
8. At which position would imidazole be protonated by acid?
9. What is imidazole antifungal?
10. Which nitrogen is more basic in imidazole?
11. Is double ring structure with an imidazole ring?
12. What is a furan ring?
13. How many pi electrons are in imidazole?

What is imidazole used for?

Imidazole is used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column. An excess of imidazole is passed through the column, which displaces the His-tag from nickel coordination, freeing the His-tagged proteins.

Which drug contains imidazole?

The commonly known imidazole compounds are clotrimazole, ketoconazole, and miconazole. Azole derivatives (e.g., fluconazole, itraconazle, voriconazole, ravuconazole, posoconazole) with 1, 2, 4-triazole ring in place of imidazole have better pharmacokinetics profile with better fungicidal activity and lower toxicity.

What are imidazole derivatives?

Many imidazole-based derivatives have been marketed as antifungal drugs such as ketoconazole (1), miconazole (2), clotrimazole (3), tioconazole (4), econazole (5), tinidazole (6), enilconazole/imazalil (7), parconazole (8), eberconazole (9), lanoconazole (10), fenticonazole (11), bifonazole (12), sulconazole (13), ...

Why is imidazole aromatic?

Imidazole - aromatic or not? ... Lastly, imidazole has 6 π - electrons (4 π- electrons from 2 π bonds and 2 π - electrons from the lone pair of electrons of -NH nitrogen atom), i.e., 4n+2 π - electrons where n = 1. Thus, imidazole is an aromatic molecule since it fulfills all of the criteria required to be one.

What is the example of Diazole?

Diazole refers to either one of a pair of isomeric chemical compounds with molecular formula C₃H₄N₂, having a five-membered ring consisting of three carbon atoms and two nitrogen atoms.

Why do we use imidazole in protein purification?

Imidazole is utilized as a competitive agent for elution of histidine-tagged proteins. In addition, imidazole can be added in low concentrations in the sample and binding buffer in order to reduce the binding of contaminant proteins, and thus increase the final purity.

Who discovered imidazole?

Imidazole was first synthesized by Heinrich Debus in 1858, but various imidazole derivatives had been discovered as early as the 1840s. His synthesis used glyoxal and formaldehyde in ammonia to form imidazole [2].

At which position would imidazole be protonated by acid?

Protonation of gaseous imidazole is predicted to occur exclusively at the N-3 imine nitrogen to yield a stable aromatic cation. Proton affinities in imidazole are calculated as 941, 804, 791, 791 and 724 for the N-3, C-4, C-2, C-5 and N-1 positions, respectively.

What is imidazole antifungal?

Among the azole group of antifungal medications, imidazoles (miconazole and ketoconazole) are commonly used for localized surface infections and triazoles (itraconazole—for dermatophytes only—fluconazole, voriconazole, and posaconazole) are used for invasive, life-threatening fungal infections.

Which nitrogen is more basic in imidazole?

Imidazole is an aromatic ring found in many biological molecules. One of its nitrogen atoms resembles that of pyrrole, and is not basic. The second nitrogen atom, which is structurally similar to the nitrogen atom of pyridine, acts as a base. However, imidazole is about 100 times more basic than pyridine.

Is double ring structure with an imidazole ring?

Definition of purines:- It is a heterocyclic aromatic organic compounds with a chemical formula of C5H4N4, it is consists of two rings pyrimidine and imidazole, it includes two of the bases in DNA and RNA, it is water soluble, purines are found in high concentration in meat and meat products, plant based diets are low ...

What is a furan ring?

Furan is a heterocyclic organic compound, consisting of a five-membered aromatic ring with four carbon atoms and one oxygen atom. Chemical compounds containing such rings are also referred to as furans. ... Furan is used as a starting point for other speciality chemicals.

How many pi electrons are in imidazole?

Imidazole is another five-membered heterocyclic ring which has 6 pi electrons and is aromatic. Both of the nitrogen atoms are sp² hybridized. One nitrogen is pyridine-like because it is part of a double bond and adds one pi electron to the aromatic pi ring.